4 Different Kinds of Pipette Tip: Standard Tips, Filter Tips, Low Adsorption Tips, and No Heat Source Tips

General introduction of the pipette tip

The pipette is an indispensable experimental instrument in biological research, and the number of its accessory tips used in the experiment is also very large. It is widely used in PCR laboratories and laboratories of hospital laboratories; third-party testing institutions, scientific research institutes, colleges and universities, pharmaceutical factories, reagent manufacturers, disease control, inspection, and quarantine.

Most of the tips on the market are made of PP, which is transparent with a wide temperature range and high chemical inertness. The difference between the high quality and low-quality tips lies in the quality of PP, whether it’s natural or recycled.

In addition, most of the tips will add a small number of additives during the manufacturing process, the common ones are:

1. Chromogenic material.
For the commonly known in the market as blue tip (1000ul) and yellow tip (200ul), the corresponding color-developing material is added to polypropylene.

2. Release agent.
The release agent helps the tip to be separated from the mold quickly after forming. It’s better not to add any additives, as the more additives there are, the greater the probability of undesirable chemical reactions occurring during pipetting. However, due to the relatively high requirements of the production process, tips that do not add additives at all are rare in the market.

Please note there are several matters when using the pipette tip.
1) When installing the tip, do not tap the tip repeatedly
For single-channel pipettes, insert the end of the pipette vertically into the tip, and gently press to the left and right to tighten; for multi-channel pipettes, align the channel with the tip and insert it at an angle, and shake it back and forth to tighten it.

When installing the tip, in order to make the pipette and the tip tightly connected, repeated impacts will first cause the tip to deform and affect the accuracy. The abrasion of the nozzle connector of the instrument will cause the air tightness between the instrument and the tip to be impaired for a long time, enter a vicious circle, and may even directly damage the instrument.

2) When tip sucking liquid, quickly loosen the lifting rod
Most instruments use a spring to generate negative pressure internally so that the liquid is forced into the tip. If the pressure difference between inside and outside increases instantaneously, it may cause liquid to flood into the instrument. Depending on the liquid inhaled, it may cause rust, corrosion, or contamination inside the instrument.

Remember: don’t go too fast, otherwise, the liquid will enter the tip too quickly and the liquid will be sucked into the pipette. When discharging liquid, press firmly on the container wall, first press to a certain height, pause for a while and then press to the second height to remove the remaining liquid.

For 5ml and 10ml pipettes, you need to immerse the tip in a 5mm liquid surface, slowly aspirate the liquid after reaching a predetermined volume. When inhaling volatile liquids, please rinse the tip 4-6 times to saturate the steam in the sleeve chamber to avoid liquid leakage.

3) When there is still liquid in the tip, do not place the pipette flat or upside down
This operation may cause a large amount of liquid to flow back into the instrument under the action of gravity, and may also cause rust, corrosion, or contamination inside the pipette, etc.

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4 different kinds of the pipette tip

Tips, as consumables used with pipettes, generally can be divided into standard tips, filter tips, low adsorption tips, and no heat source tips, etc.

1. Standard tip
The standard tip is a widely used tip. Almost all pipetting operations can use ordinary tips. This is the most affordable type of tip. It can be sterile and non-sterile

2. Filter tip
Designed for avoiding cross-contamination, the filter effectively forms a protective structure between the pipette and the sample to ensure the safety of sample aspiration; reduce the hazard of the gas residue in the gun body to the operator during the pipetting process, and improve the repeatability of the experiment; provide low-adsorption and ordinary filter tip, no coating design, will not pollute the sample, the surface of the low-adsorption tip is completely hydrophobic; can be sterilized by high temperature and high pressure. Also, the filter tip can be lengthened.

3. Low-adsorption tip
The low-adsorption tip adopts a material with a high water-blocking surface developed by modern patented technology. The made tip has a completely hydrophobic surface and has high chemical resistance. The low-adsorption tip guarantees the maximum sample recovery rate, especially when pipetting solutions containing low surface tension or other liquid detergents.

In many molecular biology techniques, the detection methods are highly sensitive and require extremely high reliability and repeatability. The reagents and samples used in DNA and protein analysis methods usually contain detergents to minimize sample adsorption. To improve the repeatability of pipetting, low-absorption tips are particularly advantageous in these applications. It applies to PCR, real-time PCR; Cloning and sequencing; Other nucleic acid processing technologies such as DNA and RNA; SDS-PAGE electrophoresis, and other protein analysis methods; Particularly suitable for handling liquids with low surface tension, such as detergents and solvents

There are some problems when using standard tips to dispense solutions containing detergents. Due to the difference in surface energy between the plastic pipette tip and the sample, the liquid will remain on the tip of the tip. Sometimes it is difficult to imagine the cause of the residue, but it will cause inaccurate pipetting and loss of valuable samples or expensive reagents. To solve this problem, use a very hydrophobic material with a low adsorption tip.

4. No heat source tip
The no heat source tip is made of transparent polypropylene, which is suitable for multi-brand pipettes. The tube wall is smooth to reduce liquid adhesion and ensure the accuracy of the transferred specimens. It is resistant to high temperatures of 121°C and can be sterilized by gamma rays. It is also specially provided without DNA enzymes. The tip is made of 100% non-recycled high-transparency polypropylene (PP) material and is made of advanced production technology. The product is straight, without bending, and has extremely high quality and precision.