HAWACH to Discuss How to Choose and Use Pipette Tips

Although the pipette tip is small, it still plays a significant role in the accuracy of our experimental results. Therefore, in order to improve our experimental results, HAWACH discusses how to choose and use the tips.

How to choose pipette tips?

Sterile filtered pipette tips are consumables that are needed to help avoid cross-contamination. Generally speaking, there is no problem when using pipette tips for forwarding pipetting operations. However, in some special situations, some pipette tips may not be suitable.

The design of some pipette tips does not fully consider the possibilities of various applications and does not leave enough space in the tips. So when you need to use reverse pipetting, which requires aspiration of the extra volume of liquid, or when the pipetting liquid is rich in protein and prone to foam, “tragedy” may happen at any time. You may find that the liquid comes into close contact with the filter element after the liquid is absorbed. As a result, pollution still has the potential to spread.

In order to control the pollution risk caused by the “close contact between the liquid and the filter element”, some have installed double filter elements in the suction head or designed a closing mechanism (when the liquid contacts the filter element, the filter element is automatically closed so that the liquid cannot be discharged), but this still cannot change the fact that precious samples are lost. The choice at this time is to buy a tip that leaves enough space in the tip to ensure that even in special applications, the possibility of liquid contact with the filter element can still be prevented.

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What is a low suction tip?

The accuracy and certainty of pipetting not only require the matching of the tip and the pipette, but also a tip suitable for the characteristics of the liquid. We often find a phenomenon in our operation. When using a standard pipette tip (PP) to pipette liquids with low surface tension (such as liquids containing detergents), it is easy to leave a film on the inner surface of the pipette tip.

The reagents and samples used in many DNA and protein analysis methods usually contain detergents. Therefore, in the experiment of this kind of application, the situation of more liquid residue is common. Liquid residues can cause inaccurate and inconsistent pipetting results, as well as the loss of some expensive samples. The development of low-absorption tips is to improve the common problem of liquid residue.

Different suppliers use different technologies to produce low-absorption tips, so their consistency, degree of hydrophobicity, and chemical resistance are different. There are two commonly used production processes for low-adsorption tips: physical polishing and chemical coating. The former uses polishing technology to treat the surface of the tip, so that the surface of the tip becomes very smooth to reduce liquid residue, and can more reliably ensure the safety of the sample.

However, the mold during polishing will age during the production process, and the consistency of the quality of the low-absorption tips cannot be guaranteed. In the chemical coating method, a layer of the hydrophobic agent is added to the surface of the tip, which may cause the risk of dissolution and introduce pollution.