How To Choose Sterile Low Retention Filtered Pipette Tip Box？
The pipetting operation runs through almost all experiments, and wrong pipetting will directly affect the experimental results and even lead to experimental failure. The pipetting operation runs through almost all experiments, and wrong pipetting will directly affect the experimental results and even lead to experimental failure. Therefore, it is necessary to choose a suitable sterile low retention filtered pipette tip box, and the calibration and maintenance of the pipette regularly and correctly.
The above process is a necessary condition to ensure the accuracy of the pipetting results. Apart from that, disposable consumables such as pipette tips are easily overlooked. It should be understood that the sterile tip is in direct contact with the liquid sample. It will not only affect the accuracy of the pipetting results but also the dissolution and the inclusion of foreign biological contaminants will directly affect the analysis results.
A micropipette with filtered pipette tips is an indispensable tool for PCR experiments. The disposable filter tip with a water barrier prevents the sample liquid from accidentally flowing into the micropipette, and reduces the possibility of cross-contamination between PCR and DNA samples during the experiment.
When choosing sterile low retention filtered pipette tip box, pay attention to the following tips
Use the sterile tip to avoid bacteria on unsterilized tips, as well as metabolites such as enzymes secreted by bacteria, which seriously contaminate experimental samples.
Making tips with poor raw materials, not 100% pure polypropylene, impurities (trace metals, bisphenol A, etc.), adding additives (plasticizers, release agents, etc.), not only the tips are transparent and shiny, but the wall thickness is inelastic (Affects the seal), and there are dissolutions that affect the experimental results.
Poorly manufactured sterile tip have inconsistent sizes, resulting in poor seal consistency, especially for multi-channel pipettes, which will cause inconsistent liquid levels and affect accuracy; poor quality suction tips the inside of the head is not smooth has flow marks and has nicks/burrs, which will cause large liquid residues and inaccurate discharge. Low retention tips are an ideal choice with minimum liquid residue and precise liquid absorption.
What is the function of the sterile pipette tip box? The box is used to protect the tip and reduce contamination. Some pipette tip box covers can also serve as a sample tank. Hawach designed and packaged to facilitate the reuse of the pipette tip box to reduce plastic waste.
Use a suitable pipette tip; in order to ensure better accuracy and precision, it is recommended that the pipetting volume is within the range of 35%-100% of the tip.
The installation of the pipette tip: In order to pursue a good seal, it is necessary to insert the pipette handle into the tip and turn it left and right or shake it back and forth to tighten it.
Pipette tip rinsing: For samples at room temperature, tip rinsing can help improve accuracy; but for high-temperature or low-temperature samples, tip rinsing will reduce the accuracy of the operation, please pay special attention to users.
Liquid aspiration speed: The pipetting operation should be kept smooth and appropriate liquid aspiration speed; too fast aspiration speed will easily cause the sample to enter the sleeve handle.
Suggest to maintain sterile low retention filtered pipette tip box
You need to check the sealing condition of the pipette regularly. In most pipettes, a layer of lubricating oil must be applied to the piston before and after a period of use to maintain the seal.
Features of sterile low retention filtered pipette tip box
It is matched with the pipette, easy to install and take, to ensure sealing; no defects, no flaws or defects on the tip shape and surface, to ensure the accuracy of pipetting; the raw materials are pure and without additives, to avoid the influence of leaching substances on the experimental results; clean and free of biological pollution, Tips should be manufactured and packaged in an ultra-clean environment.
In compliance with quality standards, tips from well-known manufacturers will have a certificate in the package to prove which tests verify the product and the verification method to ensure that any potential contamination is below the specific detection limit.