C8 Low pH HPLC Columns

Column: XChroma HPLC Column, C8-Low PH, 5μm, 4.6×250mm
Mobile Phase: A: 1.0mL TcMB, dulite in 1000mL water B:acetonitrile
Wavelength: 285nm
Temperature: 40℃
Flow Rate: 1mL / min
Injection Volume: 20μL
Notes: After every injection, need 10 column volume to get gradient equilibrium

Description of C8 Low PH HPLC Columns

Hawach Scientific Co., Ltd C8-Low pH HPLC Columns in the acidic conditions of the mobile phase have a good separation performance, stability, and longer service life. It is suitable for the separation of polarizable and objects at extremely low pH to medium pH, the pH range is 0.5-8.0.

The main reason for high performance liquid chromatography in phytochemical analysis for the shortened lifetime in low pH mobile phases is that the bonded phase is hydrolyzed under acidic conditions, which will cause significant changes in retention time and peak shape; at the same time, the hydrolysis of the bonded phase will cause LC-MS The increased detection background of the analysis affects the sensitivity and durability of the method. C8 low ph HPLC columns science equipment suppliers adopt advanced bonding and end group sealing technology to produce. Compared with columns obtained by traditional bonding technology, C8 low ph HPLC columns have better stability and reproducibility and can inhibit the hydrolysis of the bonded phase under low pH conditions.

Features of High Performance Liquid Chromatography in Phytochemical Analysis

C8-Low pH Ultra-pure all-porous silica gel, pure >99.999%
Resistant 100% aqueous phase
The C8 low PH HPLC column has better retention capability for substances with higher polarity than the C18 low PH HPLC column but does not retain as well as C18 for substances with small polarity.
Compared with C18-Low pH a slightly smaller selective provision of 300A aperture to meet large molecular biological separation needs.
Unique fixed phase bonding.
PH Range: 1.0-8.0
Particle size: 120 A, 300A for choice.

Advantages of C8-Low PH HPLC columns

The analysis of strong hydrophobic compounds is faster than the C18 column.
Good stability and low loss for LC-MS analysis.
Good peak shape can be obtained by analyzing both basic and acidic compounds.
Compatible with a low organic solvent / high water content mobile phase.
Ideal for fatty acids that are not suitable for analysis with the C18 column because of their high hydrophobicity.
Faster compounds elution reducing due to reduced hydrophobic interactions.

Applications Tell by C8 Low PH HPLC Columns Science Equipment Suppliers

Used for the determination of polypeptide and protein separation in acidic conditions.

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