HILIC Amide HPLC Columns

Column: XChromaTM HPLC Column, HILIC Amide 4.6 ×250 mm, 5µm
Mobile Phase: ACN: water =70:30
Detector: RID (40°C)
Temperature: 40°C
Flow Rate: 1.0ml/min
Injection Volume: 20μl

Description of HILIC Amide HPLC Columns

The Hilic Amide HPLC Column of Hawach is an amidic water affinity chromatography column based on super pure silica gel. The analytical column in HPLC is one of the most commonly used Hilic chromatographic columns and has good hydrophilic property through special surface modification technology. The column chromatography with controlled chemical technology ensures good repeatability of the filler between batches.

The HILIC Amide bonded phase provides a strong hydrogen bonding interaction with the stationary phase and the analyte, giving it stronger retention than other HILIC chromatograms. Analytical column in HPLC is also suitable for the separation of a variety of hydrophilic molecules, including the separation of sugars and peptide bonds.

Advantage of HILIC Amide HPLC Columns

Better stability and longer life than the Amino Acid HPLC Columns.
Excellent repeatability between batch-to-batch.
Double end-capping technology eliminates even reduces secondary reactions that may occur.
Ultra inertness and high chemical stability.
Good hydrophilic property.

Features of HILIC Amide HPLC Columns

Analysis of LC/MS for water-soluble polar compounds
As a hydrophobic analysis column
Hydrophilic polymers cover the surface of silica gel
High–polar and hydrophilic compounds

Applications of HILIC Amide HPLC Columns

Widely used in peptide, nucleotide, glycoside, hydrophobic drug analysis


Ordering Information

Item NoDescriptionPackage (pcs/pk)
SLHHA546100HPLC Column, HILIC Amide, 5μm, 120Å, 4.6x100mm1
SLHHA546150HPLC Column, HILIC Amide, 5μm, 120Å, 4.6x150mm1
SLHHA546250HPLC Column, HILIC Amide, 5μm, 120Å, 4.6x250mm1
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