How Do You Validate Pipette Tips?
When conducting research on analytical testing, a small or trace amount of liquid is generally taken with a pipette. A pipette is often used in the laboratory, with various specifications, such as single-channel pipettes or multi-channel pipettes. Such as Standard Single Channel Fixed Pipettes, Advanced Fully Autoclave 8/12 Channels Adjustable Pipettes, and Electronic Control Digital Pipettes.
Under these two kinds of pipettes, each includes a different volume, such as 0.1-2.5 μL, 0.5-10 μL, 10-100 μL, 20-200 μL, 100-1000 μL, and so on. Different specifications of pipettes use different sizes of tips, and the shapes produced by different manufacturers are slightly different, but the working principle and operation method are basically the same.
The pipette is a precision instrument. Care must be taken when using and storing it to prevent damage and affect its range. Wipe the pipette before and after each use with 75% alcohol. Soak the pipette tip in 75% alcohol for about 15 minutes once a week. If pollution occurs during use, it should be soaked at any time. If the liquid enters the sampler and causes contamination, the sampler can be disassembled and cleaned. Each area of the sampler has the identification of each area, which is treated separately when cleaning. In order to ensure the accuracy and accuracy of pipette loading and correct use of pipettes, it is necessary to calibrate the pipette regularly (six months), send it to the local measurement department for calibration, and keep the calibration records and calibration pipettes.
People are usually very cautious when buying pipettes, and are willing to spend a lot of money to buy a good brand with high precision; however, when buying pipette tips, they don’t pay enough attention, and they will buy cheap ones. Actually, the tip plays an important role in experimental results and is also an important factor that influences the pipette service life. Therefore, the choice of tips is also very important.
There are three main types of tips: ordinary tips, pipette filter tips, and low-adsorption tips. The ordinary tip has a wide range of applications. In general laboratories, ordinary tips are purchased, which are basically suitable for all pipetting. This is the most economical and cost-effective type of tip. The low adsorption tip is generally used in experiments that require high sensitivity, or precious samples or reagents that are easy to remain, which can reduce sample loss and improve recovery. The filter tip is designed to protect the pipette, prevent the liquid from being sucked back, and avoid cross-contamination during the experiment. We also provide the Pipette Tips Filters for your choice.
Pipette filter tips
Today we will focus on the pipette filter tip. The filter of the tip cartridge is loaded to ensure complete uninterrupted during the manufacturing and packaging process, featuring free of pyrogen contamination, DNase, and RNase with certifications. What’s more, all filters are pre-sterilized with radiation after packaging to enhance the protection of biological samples.
How do you validate pipette tips?
Whether in routine experiments or in today’s epidemic detection and diagnosis, frequently used pipettes and tips are crucial to the accurate quantification of experimental samples. At the same time as quantification, the filter tips we use must also meet the specifications to ensure that the samples are not contaminated.
How to test the filter tip quality? A calibrated pipette is required for testing filter tips
(1) Accuracy: Take several filter tips to be respectively sucked and weighed by a quantitative amount of pure water, and the relationship between weight and volume is converted according to 1 g/mL.
(2) Air tightness: Take several filter tips, absorb the ink containing 1% glycerin in the range, and observe that the colored liquid does not appear on the filter plug, and the liquid level is the same as qualified.
(3) Anti-aerosol performance: Take several filter tips, suck up the positive nucleic acid, blow and suck 10 times, change another new tip and blow and suck 10 times in negative pure water, and take the negative pure water as the template for amplification. If the qPCR has no amplification, it is qualified.
(4) Pollutant detection: Take several filter tips, suck negative pure water 10 times, and take negative pure water as a template for amplification. No qPCR amplification is qualified.
(5) Detection of inhibitory substances: take several filter tips, suck the weak positive quality control substance and repeat 10 times, and then extract; suck RNA nucleic acid and DNA nucleic acid repeatedly 10 times, use them as templates for amplification, qPCR amplification. The increase is not suppressed as a pass.
When to use a tip filter?
Filter pipette tips must be used in all contamination-sensitive molecular biology applications. Filter tips help reduce the possibility of smoke formation and prevent aerosol contamination, thereby protecting pipette shafts from cross-contamination and preventing PCR contamination for filter barrier preventing sample carry-over from the pipette.