How to Choose and Use Pipette Tips?

The pipette tip is the main component of the pipetting operation, and a well-adapted pipette tip is an important component of the accuracy, repeatability, and safety of the pipetting operation.

How to choose a well-matched tip?

1. Appearance inspection
A good tip (PP material), the color should be uniform and bright. Poor quality tip materials use poor materials, have variegated colors, and have poor transparency. The end of a good tip should be smooth, and the shape of the tip should be good. The tips of poor quality tips are sometimes rough, and the overall shape is even curved. Good tip, no deformation after sterilization. Poor quality tips will deform after autoclaving.

2. Airtightness inspection
Place the pipette vertically for 15 seconds after absorbing the liquid, and observe whether any droplets flow out slowly. If there is outflow, it means there is air leakage. A well-matched suction head should not leak air.

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The correct operation method of pipette tips
Aseptic operation

pinch and hold the long side of the pipetting tip tool at the same time, and take out the tip holder. Make sure that the stuck point of the removal tool is in the correct position. Insert the tip holder with pre-installed tips into a sterilized empty tip box vertically from top to bottom until it clicks into the correct position. Take the removal tool away. No need to touch the tip in all steps.

(1) Slowly and steadily loosen thumb when sucking liquid. Do not loosen it all of a sudden, in case making the plunger corroded and have air leakage.
(2) Suck the sample solution with the tip in advance and then pipette formally to achieve higher accuracy. By doing so, it would avoid too small displacement of the liquid and cause errors. As there will be a layer of “liquid film” left on the inner wall of the tip when sucking serum protein solution or organic solvent.
(3) There might be errors when the sucking liquid is in high concentration and viscosity. To solve this problem, determine the compensation amount by experiment and set the amount with the adjusting knob by changing the reading of the reading window.
(4) Measure the weight of the pure water taken with an analytical balance and calculate it to calibrate the liquid extractor.
(5) Do not hit the pipette to tighten it repeatedly. Or, the internal parts will loosen and bring damage to the pipette for long-term use.
(6) If the pipette doesn’t install a tip, do not pipette.
(7) Do not set the range over the volume, otherwise, it will be damage.
(8) Do not pipette the highly volatile, highly corrosive liquids or mixed liquid.

Advantages of HAWACH pipette tip

First of all, HAWACH pipette tip is strictly tested for proteins and proteases, which increases the credibility of the results in proteomics research. It’s stable and has no biologically active ingredients.

Secondly, HAWACH pipette tip is a clean and pollution-free tip: the product is manufactured in a fully automatic super cleanliness and dust-free workshop. Its production process complies with ISO 9001 and the pipette tip is highly pure and safe from raw material selection, clean production to automatic package.

Finally, it is an excellent comfort tip: the tip can be used with different pipettes to optimize the use effect. It can perfectly connect the nozzle of a special shape, realize the low tip installation force in the industry, reduce the repetitive labor in the pipetting process, and increase comfort. The color of the tip of different measuring ranges is different. There are three kinds of tips available, standard pipette tips, sterile pipette tips, and advanced low retentions filter pipette tips.