The tip is the main part of the pipetting operation, and the tip with good adaptability is an important component of the accuracy, repeatability, and safety of the pipetting operation. The pipette tip can be used in any molecular biology and genetics research applications, and it effectively forms a protective structure between the pipette and the sample, ensuring the safety of sample aspiration and sample distribution. How do you know which tip is right for your pipette and situation? Here is a short guide, which will help you understand your options so you can choose the right pipette tip and prevent costly experimental errors and all other issues. Hawach provided Sterile Filtered Pipette Tips and Conductive Tips for your choose from.
Influence of the tip on the experiment
The influence of the tip on the experiment mainly includes the following points: 1. The material of the tip Generally, the best tips are mostly made of 100% impurity-free polypropylene material, free of plasticizers, heavy metals, and other substances, and the wall of the tip has good elasticity. Such tips can be used in low-temperature or high-temperature environments. The endurance tolerance is also good.
2. The matching of the tip
The matching degree will affect the accuracy of the experimental results directly. The limited manufacturing process of inferior suction tips can easily lead to problems such as different tip sizes and poor sealing performance, which affect the accuracy of liquid suction. When pipetting with a discharge gun, such tips are prone to uneven liquid levels, which may lead to deviations in experimental data.
3. The structure of the tip
Inferior tips have rough inner walls, burrs, and even gaps at the ends. When the pipette is used for liquid discharge, there will be residual liquid on the inner wall of the tip, which will hang on the wall, especially for fine sample pipetting operations.
How to choose a well-matched tip?
The first consideration that comes to mind when considering which type to choose is precision and accuracy.
1. Appearance inspection
A good tip (PP material), the color should be uniform and bright. Poor quality tip materials are made of poor materials, and there will be variegated colors and poor transparency. The end of a good tip should be smooth and the tip is well-shaped. Poor quality tips sometimes have rough ends and even bend in overall shape. Good tip features no deformation after sterilization. Poor-quality tips will deform after being sterilized by high-pressure steam.
2. Air tightness inspection
Hold the pipette vertically for 15 seconds after drawing the liquid, and observe whether there is a slow flow of droplets. It means there is an air leak if outflow occurs. A well-matched tip should have no air leakage.
3. Three types of tips
Standard tips are the most widely used tips, and they are the most economical category of tips that can be used for almost all pipetting operations.
Filtered Pipette Tips
It is necessary to use filter tips in all molecular biology applications that are sensitive to pollution. Filter tips help reduce the possibility of smoke build-up, prevent aerosol contamination, and then achieve the goal of protecting the pipette shaft from cross-contamination.
Low adsorption tips
First, it can reduce the loss of precious samples caused by the need to pre-wet the tips during the test. It can be used for important procedures that require high precision for micro pipetting. Finally, it can be applied to the pipetting of samples containing molecules that are easily adsorbed on the surface of the tip, such as DNA, proteins, peptides, etc.
4. Consider the cost
Well-made, properly installed tips are critical to accuracy and ease of use. For other features (grading markers, obstacles, etc.) you should consider whether there is a price for the added features.