How To Test The PCR Tube Quality?

PCR Tube Introduction

The PCR tube is made of specially formulated polypropylene material, and the ultra-thin tube wall design is more convenient for heat transfer and quasi-control of temperature. There are two types of tube caps, protruding caps, and flat caps, to meet different experimental needs. Standard tube design can better match mainstream PCR instruments for experiments. A common PCR tube consists of a tube body and a cover body. The tube body and the cover body are connected together, and the cover body has a hollow cylindrical protrusion. It is widely used in laboratory PCR analysis, food safety testing and disease control testing, and so on.

Features of Hawach PCR Tubes

Hawach PCR tubes feature convenient and simple, accurate measurement and are easy to sterilize. There are individual and 8 strips PCR tubes available.
1. Medical grade PP material, free of RNase, DNase, exogenous DNA/RNA, endotoxin, pyrogen, the extremely low adsorption of samples and reagents, to ensure the high-temperature resistance and accuracy of the reaction system.
2. The PCR tube has the characteristics of low evaporation rate, high sealing, and convenient opening and closing of the tube cover.
3. The special interlocking process design of the tube and the tube cover has a low evaporation rate and good sealing performance. The tube cover can also be easily opened and closed, and the operation is convenient.
4. The experiment can be repeated.

thin walled 8-pcr stripe tubes PCR tubes 8 PCR stripe tubes cap

Quality Test

(1) Leak tightness: Add half the amount of ink to n PCR tubes, cover them, put them in water, and soak them for 30 minutes. If there is no ink leakage, it is qualified.
(2) Centrifugal airtightness: Add half of the n PCR tubes to pure water, put them in a centrifuge, 1000 rpm, 30 min, and the tube caps are not collapsed and no liquid leakage is qualified.
(3) Thermal airtightness: Add n PCR tubes to the half volume of pure water, cover them and weigh them, put them into the PCR machine, set up a common PCR program, and then weigh them after the program runs. The PCR tubes are not deformed. The tube cover is not broken and has no liquid leaks, and the front and rear weights are not changed to be qualified.
(4) Cold airtightness: Add half of the n PCR tubes to pure water, put them in a -20°C refrigerator for 24 hours, the centrifuge tubes are not deformed, the tube caps are not collapsed and no liquid leaks are qualified.
(5) Detection of contaminants: Add half of the negative pure water to n PCR tubes, cover them and vortex for 1 min, then let stand for 5 min, and use them as templates for amplification. No qPCR amplification is qualified.
(6) Detection of inhibitory substances: add weak positive quality control substances to n PCR tubes, let stand for 5 minutes at room temperature, and then extract; RNA nucleic acid, DNA nucleic acid, stand for 5 minutes at room temperature, use as templates for amplification, qPCR amplification Not suppressed as pass.
(7) Blank fluorescence permeability: For PCR, it is necessary to check whether the PCR tubes have non-specific fluorescent signals. Put n empty PCR tubes, cover them, put them into the PCR machine, and set up a common PCR program. After the program runs, qPCR no amplification is qualified.
(8) Permeability of positive fluorescence: For PCR, it is also necessary to check whether the PCR tube will inhibit the permeability of fluorescence. Place n PCR tubes containing weak positive samples and amplification reagents with PCR tubes confirmed to have no fluorescence interference. After the lid is closed, put it into the PCR machine to amplify together, and set up a common PCR program. After the program runs, it is qualified if the PCR amplification is not inhibited.