How To Use A Serological Pipette?

A pipette is a metering-out instrument that is used only to measure the volume of solution it releases and accurately pipettes a certain volume of solution. It is a slender glass tube with a bulge in the middle. The lower end is beak-shaped, and a marking line is engraved on the neck of the upper end, which is a sign of the exact volume removed.

The serological pipette is mainly used to accurately measure a certain volume of liquid and needs to be used with a suitable pipette. It’s suitable for tissue culture, bacterial culture, and clinical research experiments. The widely used manufacturing materials are polystyrene, glass, etc. In HAWACH, There are mainly 6 kinds of capacity specifications, namely 1.0ml, 2.0ml, 5.0ml, 10.0ml, 25.0ml, 50.0mL, except that the tube body has different precision scale marks, different capacity specifications are marked with different color rings, which is more convenient for identification and use during work; some tube ends have filter plugs, which can better prevent cross-contamination when samples are drawn.

The serological pipette can be made of glass or high-quality polystyrene (PS), and the novel design of the pipette mouth can be adapted to almost all brands of pipettes on the market. It can be sued in small volume liquid measurement and transfer, which is applied in bacteriology, cell culture clinical, scientific research, and other fields.

•The raw material is polystyrene or glass
•Independent paper and plastic packaging
•Manufactured in 100,000 cleanroom
•Negative scale for extra volume
•Polyolefin filter element to prevent contamination
•Highly dropper-free tip design
•Slim profile enables ergonomic conditions inside the sterile hood
•In addition to the different precision scale marks on the tube body, different specifications with different colors for easy identification
•Each package has an independent item number and batch number identification, which is convenient for quality tracking and traceability

The correct use of methods and steps of the serological pipette
1. Before using: Before using a serological pipette, first look at the pipette marking, accuracy level, and the position of the scale mark.

2. Liquid suction: Pinch the upper end of the pipette with the thumb and middle finger of the right hand, and insert the lower mouth of the pipe into the solution to be sucked. The insertion should not be too shallow or too deep, generally, 10~20mm, too shallow will produce Aspirate and suck the solution into the ear bulb to stain the solution, too deep and the solution will adhere to the outside of the tube too much. Take the ear-washing bulb in the left hand, connect it to the upper mouth of the tube and slowly inhale the solution, first inhale about 1/3 of the capacity of the tube, hold the mouth of the tube with the index finger of the right hand, take it out, hold it horizontally, and turn the tube to make the solution come into contact with it. Place above the scale to replace the water on the inner wall, and then release the solution from the lower mouth of the tube and discard it. After repeated washing 3 times, the solution can be sucked up to about 5mm above the scale, and immediately press to the mouth of the tube with the index finger of the right hand.

3. Adjust the liquid level: lift the pipette up to leave the liquid level, wipe off the liquid stuck on the outer wall of the serological pipette with filter paper, the end of the pipe is against the inner wall of the solution container, the pipe body is kept vertical, and the index finger is slightly relaxed. Make the solution in the tube slowly flow out from the lower mouth until the bottom of the meniscus of the solution is tangent to the marking line, and press the tube mouth immediately with the index finger. Remove the droplet against the wall, remove the pipette, and insert it into the vessel that holds the solution.

4. Release the solution: If the container for the solution is a conical flask, the conical flask should be tilted 30°, the disposable serological pipette should be kept vertical, the lower end of the pipe should be close to the inner wall of the conical flask, loosen the index finger, and let the solution slowly move along the bottle wall. Slowly flow down, when the liquid level drops to the discharge head and the tube touches the inner wall of the bottle for about 15 seconds, then remove the pipette. A small amount of solution remaining at the end of the tube cannot be forced to flow out with strong external force, because it has been taken into account when calibrating the volume of solution remaining at the end.