What Precautions Should Be Taken While Using a Pipette

The pipette tip with filter element is made of ultra-pure raw materials and is highly hydrophobic, which can perfectly block aerosols and prevent fluid and liquid vapor from contaminating the inside of the pipette. As we know, to detect the virus would require a pipette with filtered tip. Viruses are contagious. If not isolated during the virus detection process, the pipette will be transmitted with the virus. The test samples are different, and the filter tip can prevent cross-contamination of samples during the pipetting process.

The pipette with filtered tips have many advantages, like reducing the hazard of the gas residue in the pipette body to the operator during the pipetting process, and improving the repeatability of the experiment; it could be a protective structure to ensure the safety of sample aspiration between the pipette and the sample.

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Use notes for pipette with filtered tips

Here are some notes for the use of pipette with filtered tips.
1. Before use: When using a pipette, we must first look at the pipette mark, accuracy level, and position of the scale mark.

2. Suction: Pinch the upper end of the pipette with the thumb and middle finger of your right hand, and then insert the lower mouth of the pipe into the solution to be absorbed. The insertion should not be too shallow or too deep, generally 10-20mm, too shallow will produce aspirate and suck the solution into the ear wash ball to soil the solution. If it is too deep, too much solution will be attached to the outside of the tube.

Take the ear-washing ball in the left hand, connect it to the upper mouth of the tube, and slowly inhale the solution. First, suck in about 1/3 of the volume of the tube, press the mouth of the tube with the index finger of your right hand, take it out, hold it horizontally, and turn the tube so that the solution touches the part above the scale to replace the water on the inner wall, and then release the solution from tube lower mouth and discard it. Repeat this way 3 times. After that, we can draw the solution above the scale, and immediately press the mouth of the tube with the index finger of the right hand.

3. Liquid level adjustment: Take the pipette up and leave the liquid surface, with the pipette end still against the inner wall of the solution container, and keep the tube body upright. Slightly relax the index finger (sometimes rotating the pipette slightly) so that the solution in the pipette slowly flows out of the lower opening, until the bottom of the menus of the solution is tangent to the line, immediately press the opening of the pipette with the index finger. Remove the tip of the drop against the wall, remove the pipette, and insert it into the container that receives the solution.

4. Release the solution: If the container receiving the solution is an Erlenmeyer flask, the Erlenmeyer flask should be tilted 30° while keeping the pipette upright, and the lower end of the tube should be close to the inner wall of the Erlenmeyer flask. Loosen your index finger slightly to allow the solution to slowly flow down the wall of the bottle. After the flow is completed, the tip of the tube touches the inner wall of the bottle for about 15 seconds, and then the pipette is removed. At the end of the tube, there would be a small amount of residual solution which can not be flowed out by external force, because the volume of solution retained at the end of the tube has been taken into account.