About the Features, Classifications and Use Notes for Centrifuge Tubes
As a frequently used experimental tools, the centrifuge tube can be used for liquid components stratification and separation in the tube. Centrifuge tubes are suitable for handling samples conveniently and safely. Today, HAWACH will discuss the centrifuge tubes features, classifications and use notes. Weclome to contact HAWACH for more specific trouble shootings.
Features of centrifuge tubes
Made of high-quality transparent polymer material polypropylene (PP), HAWACH centrifuge tubes have been widely used in a variety of experimental operations to meet the requirements of bioanalytical grades.
1. Two types of tube bottoms are available: self-supporting and conical bottom.
2. The sealing cover has a sealing ring, which has good sealing performance.
3. The inner and outer surfaces of the tube are smooth and the color is uniform.
4. The outer surface is printed with a clear black scale and a white area where text can be written.
5. Strictly tested for leaks.
6. It can effectively deal with some difficult components in soil samples such as: eubacterial spores, endospores, Galanz-positive bacteria, yeast, nematodes, seaweed, fungi, etc.
7. In the process of sample processing, nucleic acid can be effectively protected throughout the extraction process, and RNA contamination can be reduced to a greater extent.
8. The purification method based on silica beads can effectively remove PCR inhibitors such as humic acid and polyphenols
9. The purified DNA can be directly used in subsequent experiments such as PCR and enzyme digestion.
10. For samples with particularly high humic acid content, additional treatment methods are added.
The centrifuge tubes can be divided in to different types based on different standards. Firstly, from the volume, it can micro-centrifuge tubes (1.5 ml and 2 ml available), ordinary centrifuge tubes (50mL, 15mL) and centrifuge bottles with 250 ml and 500 ml. Secondly, it can divided into conical bottom, which is the most used types of centrifuge tubes, flat-bottom centrifuge tubes, and round-bottom centrifuge tubes, based on the centrifuge tubes bottom shapes. Thirdly, the centrifuge tubes can be classified based on the cap closure, centrifuge tubes sealed by pressing, micro centrifuge tubes and screw cap centrifuge tubes, which can be divided into flat caps and plug caps, with a plug shape on cap top. For the screw cap centrifuge tubes, there is an overall capacity indication with a finer scale.
Also, the centrifuge tubes can be divided by material, like glass, steel and plastic.
1. Glass centrifuge tube: When using glass tubes, the centrifugal force should not be too large. A rubber pad should be used to prevent the tubes from breaking. For high speed centrifuges, glass tubes will not be chosen. The consequence of spillage is to contaminate the rotor and centrifugal chamber, affecting the normal operation of the inductor. During ultracentrifugation, the centrifuge tube must be filled with liquid, because ultracentrifugation requires a high vacuum, and the centrifuge tube can be prevented from being deformed only by filling it up.
2. Steel centrifuge tube: The steel centrifuge tube has high strength, no deformation, heat resistance, frost resistance and chemical corrosion resistance. Its application is also quite extensive, but it should also avoid contact with strong corrosive chemicals, such as Strong acids, strong bases, etc. Try to avoid corrosion from these chemicals.
3. Plastic centrifuge tube: Plastic centrifuge tubes are used more, and can be divided into PP, PC, PS, etc. There are different plastic materials centrifuge tubes available, for different needs. With samll hardness, and transparent or translucent appearance, we can take out the sample by puncture. But there are also shortcomings, like short service life, poor corrosion resistance to organic solvents and easy to deform etc. We should consider polypropylene plastic centrifuge tubes as much as possible when selecting plastic centrifuge tubes.
The function of the tube cover
All plastic centrifuge tubes have caps, and the caps must be tightly closed to prevent liquid leakage.
Here are three important roles of the cap:
1. When used for radioactive or corrosive samples, prevent the sample from leaking.
2. Prevent the sample from volatilizing.
3. Support the centrifuge tube to prevent deformation of the centrifuge tube.
Matters needing attention
1. When selecting plastic centrifuge tubes, consider polypropylene PP plastic centrifuge tubes as much as possible. Please pay attention to check whether the tube cover is tight, and whether it can be tightly covered during the test, so as to achieve the inversion without leakage.
2. Do not put any substance on the centrifuge cover. Be sure to clean the inner cavity after each use.
2. When using the centrifuge tube, do not use one tube for multiple times. Please pay attention to the volatilization of the sample and the leakage of some radioactive or corrosive samples; during the storage process, it should be sealed well; otherwise, deformation occurs during use.