NH2 HPLC Columns

Column: XChroma HPLC Column, NH2, 5μm, 4.6×150mm
Wavelength: 210nm
Mobile Phase: Acetonitrile/phosphate buffer = 70/30
Temperature: 35℃
Flow Rate: 1.8mL/min
Injection Volume: 20μL

Description of NH2 HPLC Columns

Hawach NH2 stationary phase of HPLC Column is based on ultra pure porous spherical silica gel matrix, by using the HPLC column stationary phase bonding technology, using the contain aminopropyl functional groups of organic silane bonded, can faster than silica gel column balance, is sensitive to the water content of mobile phase without silica gel column. Most of the time, NH2 HPLC columns are used in normal phase LC analysis.

NH2 HPLC columns are classified as normal phase chromatography according to the HPLC column stationary phase matrix you can get:
-Quality assurance brought by the world’s only monodisperse microsphere packing, higher column efficiency, better result stability, and longer column life
-Multiple retention mechanisms, suitable for normal phase, reverse phase, and ion exchange separation at the same time
-Reverse phase mode separation of hydrophilic and polar compounds, such as carbohydrates and monosaccharides, oligosaccharides, sugar alcohols, and other sugar compounds
-Separate acids, hydrocarbons, vitamins A and D in normal phase mode

Advantage

End-capping technology ensures high chemical stability.
More uniform aperture because of the unique silica gel matrix treatment technology.
Reasonable sample size & pore size make the good flow rate.
Peak volume is high and peak shapes are perfect.
Ultra inertness, compatible with a wide range of HPLC instruments.
The separation rate is reasonable.
The influence of raw material silicone is greatly reduced.
pH ranges widely with a long service life

Features of NH2 HPLC Columns

For applications in aggressive normal phase mode with an aqueous eluent
Can separate Vitamins A and D in the normal-phase mode
Carbohydrates and monosaccharides can be separated in the reversed-phase mode on NH2, but are not suited for the separation of compounds and sugars that contain aldehyde, carbonyl
Able to separate some negatively charged molecules for weak anion exchange mechanism in the low pH buffer solution.

Applications of NH2 HPLC Columns

1. NH2 HPLC columns are widely used in the analysis of low molecular weight sugars such as glucose, fructose, xylose, and lactose.
2. Suitable for most of the positive phase chromatography applications
3. Normal phase analysis, weak anion exchange, and reversed-phase analysis of water containing polar compounds
4. Positive, reverse, weak anion exchange

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